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Titlebook: Clinical Applications of PCR; Rajyalakshmi Luthra,Rajesh R. Singh,Keyur P. Patel Book 2016Latest edition Springer Science+Business Media N

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High-Resolution Melt Curve Analysis in Cancer Mutation Screen,assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.
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Ethnicity and Geopolitics of Rohingya Crisisnrich, and whenever a mutation is suspected to be present but is undetectable using conventional PCR and sequencing methods. We describe in this chapter our COLD-PCR-based pyrosequencing method for . mutation detection in various clinical samples using DNA extracted from either fresh or fixed paraffin-embedded tissue specimens.
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Michael Blain,Angeline Kearns-Blainon Sphere™ Particles (ISPs) on the Ion Personal Genome Machine. (PGM™) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouch™ System that includes the Ion OneTouch™ 2 and the Ion OneTouch™ ES instruments for template generation and enrichment of template-positive ISPs, respectively.
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Reconceptualised Security in MexicoR and single molecular emulsion PCR for genotyping, gene expression, and miRNA expression. In this chapter, the use of a microfluidics-based PCR for simultaneous screening of 14 known fusion transcripts in patients with leukemia is described.
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