Titlebook: Clinical Applications of Mass Spectrometry in Drug Analysis; Methods and Protocol Uttam Garg Book 2024Latest edition The Children�s Mercy H

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Mass Spectrometry in Clinical Laboratory: Applications in Therapeutic Drug Monitoring and Toxicologo present “off-the-shelf” and “ready-to-use” protocols of recent developments in new assays to help the clinical laboratory community adopt the technology and analysis for the betterment of patient care.

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Quantitative LC-MS/MS Analysis of Amphetamines and Cocaine in Human Urine,t response for each analyte at a given concentration is normalized against stable isotope-labeled internal standard. In addition, the assay can be multiplexed across more than one LC channel to obtain high-sample throughput.

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Quantitative LC-MS/MS Method for the Simultaneous Measurement of Six Antiepileptics and Pentobarbittopiramate, zonisamide) and that of pentobarbital by LC-MS/MS. Liquid-liquid sample extraction is followed by reversed-phase chromatography using biphenyl HPLC column and gradient elution. Two MRM transitions are monitored for each drug, and their heavy isotope labeled internal standards. Six-point

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Analysis of Barbiturates in Urine by LC-MS/MS, method helps gain efficiency in a high-volume laboratory environment. Additional features of this protocol that help in efficiency gain are automated extraction using Hamilton™ liquid handling system and algorithmic data review using Ascent™ software.

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Determination of Busulfan and Melphalan in Plasma by Turbulent Flow Chromatography-Tandem Mass Speccted from plasma with methanol containing deuterated internal standards. Turbulent flow chromatography coupled with reversed-phase HPLC was used for separation, while the mass spectrometer was set in the positive ion mode. This method has proven accurate and rapid and allowed for timely dose adjustm

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https://doi.org/10.1007/3-7908-1668-Xwith a bias of −0.2–5%. The intra- and inter-day imprecision were less than 5.0% for all the analytes. The linear assay ranges were 0.3–26 μg/mL for GPN, 0.15–24 μg/mL for LCM, 7.4–1881 ng/mL for PER, 0.03–13 μg/mL for PRG, 0.78–90 μg/mL for RFM, and 0.3–43 μg/mL for VGB.

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