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Titlebook: Clinical Applications of Capillary Electrophoresis; Methods and Protocol Terry M. Phillips,Heather Kalish Book 2013 Springer Science+Busine

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发表于 2025-3-21 17:59:55 | 显示全部楼层 |阅读模式
书目名称Clinical Applications of Capillary Electrophoresis
副标题Methods and Protocol
编辑Terry M. Phillips,Heather Kalish
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Clinical Applications of Capillary Electrophoresis; Methods and Protocol Terry M. Phillips,Heather Kalish Book 2013 Springer Science+Busine
描述.Capillary electrophoresis (CE) is a powerful and rapid tool for performing complex analyses of a number of different molecular species ranging from small inorganic ions to large nucleic acid fragments and proteins. It is quickly becoming established as a useful tool in clinical medicine due to its consumption of minute samples (less than a microlitre), low reagent costs, and extreme sensitivity, depending upon the source of detection used.  .Clinical Applications of Capillary Electrophoresis. aims to give an in-depth manual of CE applications in several important areas of clinical science.  Divided into seven sections, this volume provides a brief overview of how CE has been applied in clinical settings, followed by several chapters on CE analysis of important diagnostic molecules and biofluids, as well as descriptions of applications in clinical chemistry, hematology, bacteriology, virology, disease-associated biomarker discovery, immunology and genetic analysis. Written in the successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protoc
出版日期Book 2013
关键词bacteriology; capillary electrophoresis; clinical chemistry; disease biomarker discovery; hematology; imm
版次1
doihttps://doi.org/10.1007/978-1-62703-029-8
isbn_softcover978-1-4939-6238-9
isbn_ebook978-1-62703-029-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2013
The information of publication is updating

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Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contacts. Most of these analyses employ long separation pathways. This chapter describes CE/C.D determinations of 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5–10 mol/L acetic acid. The time of analy
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Development of an On-Site Measurement System for Salivary Stress-Related Substances Based on Microchsystem. A rapid point-of-care testing platform for stress measurement based on immunoassay and capillary electrophoresis (CE) separation was ­developed using a poly(methyl methacrylate) (PMMA) chip and a laboratory-built microchip CE system. A method for the quantitative determination of sIgA in hum
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Simultaneous Determination of Atenolol and Amiloride by Capillary Electrophoresis with Capacitively e β-blocker drugs (atenolol and amiloride) in pharmaceutical formulations. 150 mM acetic acid was used as background electrolyte. The influence of several factors (detector excitation voltage and frequency, buffer concentration, applied voltage, capillary temperature, and injection time) was studied
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A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Deteoreaction of T4 and horseradish peroxidase (HRP)-labeled T4 (HRP–T4) with anti-T4 mouse monoclonal antibody (Ab). HRP–T4 and the HRP–T4–Ab complex were separated and quantified by using microchip electrophoresis (MCE) with chemiluminescence (CL) detection. The MCE separation was accomplished within
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High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics SystemsHowever, complex and time-consuming protocols are often necessary to obtain suitable samples for analysis. We here describe a short and robust assay to prepare 8-aminopyrene-1,3,6-trisulphonic acid-labeled N-glycans from serum samples. It only involves the subsequent addition of reagents and incubat
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Screening of Matrix Metalloproteinase Inhibitors by Microanalysis with Fluorescence Detectionions in an offline mode, but the reaction can also be performed online, inside the capillary, where the reaction products are formed and analyzed. In this way, an integrated setup is obtained which allows a higher degree of automation and miniaturization in analytical systems. This chapter presents
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