Titlebook: Citrus Tristeza Virus; Methods and Protocol Antonino F. Catara,Moshe Bar-Joseph,Grazia Licciar Book 2019 Springer Science+Business Media, L

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Amplification and Cloning of Large cDNA Fragments of the , Genome, CTV obtained from two RT-PCR amplified products. These 5′- and 3′-genomic halves, which were designed to be overlapped in 15 nt in their 3′- and 5′-ends, respectively, were used as templates for further overlapped PCR to amplify the entire ~20 kb CTV genome. The resultant full cDNA PCR product was

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1064-3745 rologists, molecular biologists, and graduate students interested in performing qualitative and quantitative tests as well as recently-developed diagnostic methods in order to find solutions to improve the management of this disease..978-1-4939-9560-8978-1-4939-9558-5Series ISSN 1064-3745 Series E-ISSN 1940-6029

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R. A. Adey,R. Harfield,C. A. Brebbiater, tissue-printing and squashing capture methods for direct sample preparation without extract preparation or nucleic acid extraction and purification were coupled with validated real-time RT-PCR detection protocols based on TaqMan chemistry for CTV detection.

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Kai-Yuan Cai,Chuan-Yuan Wen,Ming-Lian Zhangully applied for rapid and sensitive detection of . (CTV) by using four oligo-primers, targeting a conserved coat protein gene (CPG) of an Indian CTV isolate. The result of assay is visible in naked eyes easily in the presence of SYBR Green I (100×) or on 1.5% agarose gel electrophoresis. CTV-RT-LAM