Titlebook: Citrus Tristeza Virus; Methods and Protocol Antonino F. Catara,Moshe Bar-Joseph,Grazia Licciar Book 2019 Springer Science+Business Media, L

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Bioinformatic Tools and Genome Analysis of ,and the study of host-pathogen interactions. Virus detection has been an important application of this technology, which has resulted in the discovery of novel viruses or viral strains as well as for the detection of known viruses in a plant sample. Here we describe the entire process that needs to

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: Host RNA Silencing and Virus Counteraction,d by the virus via its three RNA silencing suppressors (RSS), the small RNAs (sRNAs) of three virus-host combinations were deep sequenced. The subsequent analysis indicated that CTV sRNAs (1) constitute more than half of the total sRNAs in the susceptible Mexican lime and sweet orange, while only 3.

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Proteomic Response of Host Plants to ,roteins to obtain two-dimensional gels with a large number of spots, well distributed, and with good quality. The extraction process that associates successive washes with ultrasonication steps proved to be efficient for the detection of proteins. With this technique it was possible to obtain clear

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,Gene Expression in Citrus Plant Cells Using Helios® Gene Gun System for Particle Bombardment,teins. However, due to technical limitations, so far these studies have been limited to the nonnatural host ...Particle bombardment is a physical method to deliver nucleic acid and other biomolecules into the cells directly. The Helios. gene gun (Bio-Rad, Hercules, CA) is a handheld device that uses

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Book 2019, a member of the genus .Closterovirus., family .Closteroviridae.. Thanks to the highly sensitive and specific diagnostic procedures developed, knowledge of the molecular characteristics, expression strategies, genetic variability, and epidemiology of the virus has improved significantly, as this vo

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