Titlebook: Circadian Regulation; Methods and Protocol Guiomar Solanas,Patrick -Simon Welz Book 2022 The Editor(s) (if applicable) and The Author(s), u

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Irene Ring,Bernd Klauer,Bengt Å. Månssoning of several consecutive steps in which organotypic tissue cultures or cells can be used. Our approach targets the potential resetting mechanism at three levels: the hormone, the direct clock gene target, and the tissue clock response.

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Irene Ring,Bernd Klauer,Bengt Å. Månssonpheroids to simulate in vivo tissue integrity, as well as to increase complexity of cell–cell interactions and the abundance of potential coupling factors. Circadian rhythms are monitored using live-cell imaging of spheroids equipped with circadian reporters over several days.

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In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microsmodel of acute lung injury. The method allows four-dimensional capture and quantification of NET formation dynamics over time and should be a useful resource for those interested in visualizing neutrophil responses in vivo.

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Book 2022d cutting-edge, .Circadian Regulation: Methods and Protocols .aims to be a useful practical guide to researches to help further their study in this field. .Chapters 3, 4, 8, and 17  are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com..

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