Titlebook: Circadian Regulation; Methods and Protocol Guiomar Solanas,Patrick -Simon Welz Book 2022 The Editor(s) (if applicable) and The Author(s), u

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Regional Stratigraphy of North Americae to structure-based drug design studies. Here, we describe virtual screening methods that can be utilized for the identification of small molecules regulating the activity of core clock protein Cryptochrome 1.

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Terence J. Centner,Lewell F. Gunterhigh-throughput capability and spatial resolution. It can be readily adapted to a wide variety of applications, such as different sample types and plate sizes, under a wide range of physiologically relevant conditions.

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Olfa Manai Daboussi,Amel Hedhliircadian clock mechanism, and can be great therapeutic candidates. Here we describe the detailed methods of measuring cellular circadian rhythms in a high-throughput manner for chemical screening to identify compounds that affect circadian rhythms by targeting clock-related proteins.

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Terence J. Centner,Lewell F. Gunterlysis and metabolic studies. In this chapter, we describe detailed methodology for isolation of viable primary hepatocytes, techniques for culturing these cells, methods for synchronization of the circadian clock, transfection and luciferase reporter analysis, as well as glucose production assays as a functional readout of metabolic state.

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