Titlebook: Cilia; Methods and Protocol Vito Mennella Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Sc

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Book 2024f different ciliated tissues and their wide applicability in cilia biology. Chapters in this book primarily focus on methods to study multiciliated cells, and discuss topics such as SARS-CoV-2 infections of human primary nasal multiciliated epithelial cells; expansion microscopy of ciliary proteins;

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AORTA

Regierungssysteme in Mittel- und Osteuropaodified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).

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Immunolabel-First-Expand-Later Expansion Microscopy Approach Using Stable STED Dyes,ing their use in an immunolabel-first-expand-later approach. Our protocol overcomes the frequent technical limitation of poor, unreproducible binding of primary antibodies to proteins after denaturation. We demonstrate the applicability of this approach by analyzing both a centriole appendage protein Cep164 and a ciliary protein ARL13B.

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Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance,odified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).