Titlebook: Chronic Lymphocytic Leukemia; Methods and Protocol Sami N. Malek Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia,):2313–2324, 2017). It is important to note that immunoblotting should always be combined with other quantitative methods such as flow cytometry to confirm activation of these signaling pathways (Aguilar-Hernandez et al. Blood 127(24):3015–3025, 2016).

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The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-Mutant CLL,ve allowed development of isogenic models and detailed analysis of changes to the transcriptome with relative ease. In this manuscript, we focus on two relevant methods to study splicing factor mutations in CLL: development of isogenic scalable cell lines and informatics analysis of RNA-Seq datasets.

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Ex-Vivo Signal Transduction Studies in Chronic Lymphocytic Leukemia,th protein and chemical methods is crucial if we are to investigate and correlate biological changes with therapeutic responses in patients. Herein, we describe the use of western blotting also referred to as immunoblotting as a method that can semiquantitatively evaluate changes in protein expressi

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Ex Vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells,e the first agent is administered to patients and second drug is tested in these patients’ cells in vitro. These assays have been effective in identifying novel agents that work additively or synergistically. In this chapter, we provide a step-by-step protocol for ex vivo drug testing that can be us