Titlebook: Chronic Lymphocytic Leukemia; Methods and Protocol Sami N. Malek Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 20

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Gene Disruption Using CRISPR-Cas9 Technology,n a specific and efficient manner. The Type II CRISPR-Cas9 system enables gene editing by directed DNA cleavage followed by either non-homologous end joining (NHEJ) or homology-directed repair (HDR). Here, we described the use of the Type II CRISPR-Cas9 system in detail from designing the guides to analyzing the desired gene disruption events.

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Rare Variant Quantitation Using Droplet Digital PCR,an enumerate both mutant and wild-type alleles enabling highly sensitive detection and quantitation of the variant allele frequency of mutated genes. In this protocol, we describe a method for using ddPCR to detect mutations in genomic DNA with a sensitivity of up to 1 in 50,000 DNA copies.

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Methods for Measuring ctDNA in Lymphomas,tary source of tumor DNA to tissue biopsy for genotyping. Applications of cfDNA analysis in lymphomas include: (1) identification of tumor mutations in a biopsy-free manner; (2) tracking tumor clonal evolution and identification of mutations causing resistance to treatment; and (3) monitoring of residual disease after therapy.

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Sami N. MalekInclude cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implemenation advice from the experts

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/226392.jpg

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