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Titlebook: Chromosome and Genomic Engineering in Plants; Methods and Protocol Minoru Murata Book 2016 Springer Science+Business Media New York 2016 pl

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发表于 2025-3-21 19:13:35 | 显示全部楼层 |阅读模式
书目名称Chromosome and Genomic Engineering in Plants
副标题Methods and Protocol
编辑Minoru Murata
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chromosome and Genomic Engineering in Plants; Methods and Protocol Minoru Murata Book 2016 Springer Science+Business Media New York 2016 pl
描述.This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study... .
出版日期Book 2016
关键词plant genetics & genomics; DNA insertion; plant chromosome vector; plant artificial chromosomes; Cre/Lox
版次1
doihttps://doi.org/10.1007/978-1-4939-4931-1
isbn_softcover978-1-4939-8163-2
isbn_ebook978-1-4939-4931-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
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Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase,to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756–1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Wherea
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Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking,ly stack DNA molecules in vitro and that the in vitro derived gene stack can be incorporated into an . transformation vector by in vitro recombination. After transfer to the chromosome by Agroinfection, the transgenic locus harbors a new target site that can be used for the subsequent in vivo stacki
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CRISPR/Cas-Mediated Site-Specific Mutagenesis in , Using Cas9 Nucleases and Paired Nickases,ng the target sequence and its applicability to organisms throughout all kingdoms of life. The need for an easy-to-use and reliable nuclease is especially high in plant research, as precise genome modifications are almost impossible to achieve by .-mediated transformation and the regeneration of pla
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Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination,spectively, allows enrichment of cells harboring target genes modified via gene targeting (GT). We have developed a successful GT system employing positive–negative selection and subsequent precise marker excision via the . transposon derived from the cabbage looper moth to introduce desired modific
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