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Titlebook: Chromosome and Genomic Engineering in Plants; Methods and Protocol Minoru Murata Book 2016 Springer Science+Business Media New York 2016 pl

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https://doi.org/10.1007/978-1-4020-6979-6on mechanisms leading to such genomic rearrangements were analyzed. The rearrangements were induced by sister chromatid transposition (SCT), homologous recombination (HR), and single chromatid transposition (SLCT). Among them, 22 events (65 %) were found to be transmitted to the next generation. The
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Mary Ann Cooper,Ronald L. Holle a series of dots. The size of DNA in the dot is estimated to be approximately 1 kb, it corresponding to the resolution of fiber FISH. This makes it possible to analyze structures of transgenes on DNA fibers in detail.
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Minoru MurataIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
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Appendix: Decomposition with Differencingminichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing transgenes and telomere sequences concurrently to the cell to truncate an endogenous chromosomal target. Two methods can be utilized; either the telomere sequences can be incorporated into a binary vector
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Appendix: Decomposition with Differencingly stack DNA molecules in vitro and that the in vitro derived gene stack can be incorporated into an . transformation vector by in vitro recombination. After transfer to the chromosome by Agroinfection, the transgenic locus harbors a new target site that can be used for the subsequent in vivo stacki
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https://doi.org/10.1007/978-1-4020-6979-6letional mutagenesis, as well as for the generation of artificial ring chromosomes in model plants such as Arabidopsis and tobacco. However, it takes a long time to complete this process, even in Arabidopsis. To overcome this issue, a new binary vector, pDLHC, has been developed to induce chromosoma
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