Titlebook: Chondrocytes; Methods and Protocol Tariq M. Haqqi,Véronique Lefebvre Book 2021 Springer Science+Business Media, LLC, part of Springer Natur

药物

Chun-Tao Liu,Peng Li,Shu-Tian Zhangold standard approaches to induce postnatal chondrocyte-specific gene modifications include the Cre-loxP and Tet-ON/OFF systems. Selecting the appropriate promoter/enhancer sequences to drive Cre expression is of crucial importance and determines the specificity of conditional gain- or loss-of-funct

DEFT

Herbivorous

Sorting, Searching, and Merging,ination of TRIzol. reagent and spin column chromatography (Norgen Total RNA Purification Kit) was the best approach to generate higher quality RNA. Here, the average RNA Integrity Number (RIN), as determined by Bioanalyzer technology, was 7.1. We then applied this method to attempt to isolate RNA di

HEW

monopoly

Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse,old standard approaches to induce postnatal chondrocyte-specific gene modifications include the Cre-loxP and Tet-ON/OFF systems. Selecting the appropriate promoter/enhancer sequences to drive Cre expression is of crucial importance and determines the specificity of conditional gain- or loss-of-funct

Liberate

Isolation of Mouse Growth Plate and Articular Chondrocytes for Primary Cultures, from neonatal and adult mice, respectively. Both methods involve manual and enzymatic procedures to clean cartilage samples from contaminating tissues and to release chondrocytes as single-cell suspensions from their cartilage matrix.

Osteoarthritis

RNA Isolation from Articular Cartilage Tissue,ination of TRIzol. reagent and spin column chromatography (Norgen Total RNA Purification Kit) was the best approach to generate higher quality RNA. Here, the average RNA Integrity Number (RIN), as determined by Bioanalyzer technology, was 7.1. We then applied this method to attempt to isolate RNA di

羽毛长成

1064-3745 aim of sparking new research ideas in order to solve many of the pending mysteries regarding cartilage biology, disease mechanisms, and potential treatments.978-1-0716-1121-0978-1-0716-1119-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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苦涩

Protocol for the Isolation of Intact Chondrons from Healthy and Osteoarthritic Human Articular Cart). They have attracted attention as a more physiological and biomimetic in vitro model for evaluating chondrocyte function and metabolism as compared to single chondrocytes. Chondrons may be more suitable for in vitro studies than primary chondrocytes that have been isolated without PCM since their