Titlebook: Chondrocytes; Methods and Protocol Tariq M. Haqqi,Véronique Lefebvre Book 2021 Springer Science+Business Media, LLC, part of Springer Natur

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Cartilage-Specific Cre Recombinase Transgenes/Alleles in the Mouse,everal forms, including growth plate and articular cartilage, wherein chondrocytes follow a differential differentiation program and have different fates. The induction of gene modifications in cartilage specifically relies on mouse transgenes and knockin alleles taking advantages of transcriptional

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Fetal Growth Plate Cartilage : Histological and Immunohistochemical Techniques,l progenitors condense and transdifferentiate directly into osteoblasts, giving rise to the flat bones of the skull. The majority of the skeleton develops through endochondral ossification, in which mesenchymal progenitors give rise to a cartilaginous template that is gradually replaced by bone. The

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Laser Capture Microdissection of Mouse Growth Plate Cartilage,olves spatial regulation of signaling pathways and interactions between cells with different functions, expression profiles, and lineages. Here, we focus on assessing the growth plate cartilage, composed of multiple functionally and histologically distinct zones, to investigate temporally and spatia

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Small Nucleolar RNA Expression Profiling in Cartilage,lar RNAs (snoRNAs) direct chemical modification of other RNA substrates and are involved in endoribonucleolytic pre-rRNA processing. They have thereby a role by fine-tuning spliceosome and ribosome function and can thus accommodate changing requirements for protein synthesis in OA. Here we describe

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ChIP-Seq Assays from Mammalian Cartilage and Chondrocytes,matin regulators as well as histone modification patterns in a genome-wide manner. ChIP-seq consists of five major steps: (1) preparation of cells and chromatin, (2) ChIP, (3) ChIP-seq library construction, (4) sequencing of ChIP DNA with a next-generation sequencer (NGS), and (5) computational anal