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Titlebook: Chloroplast Biotechnology; Methods and Protocol Pal Maliga Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer N

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发表于 2025-3-21 17:10:16 | 显示全部楼层 |阅读模式
书目名称Chloroplast Biotechnology
副标题Methods and Protocol
编辑Pal Maliga
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chloroplast Biotechnology; Methods and Protocol Pal Maliga Book 2021Latest edition Springer Science+Business Media, LLC, part of Springer N
描述This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Cutting-edge and practical, .Chloroplast Biotechnology: Methods and Protocols, Second Edition. is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights .of applications in agriculture, industrial biotechnology and healthcare.  .
出版日期Book 2021Latest edition
关键词RNA Polymerases; Unicellular Algae; Nicotiana tabacum; Polyethylene Glycol Treatment; Real-Time PCR
版次2
doihttps://doi.org/10.1007/978-1-0716-1472-3
isbn_softcover978-1-0716-1474-7
isbn_ebook978-1-0716-1472-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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发表于 2025-3-21 22:25:01 | 显示全部楼层
Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression about the factors influencing the efficiency of plastid transgene expression, and highlight possible optimization strategies to alleviate problems with poor expression levels. I also discuss available techniques for inducible expression of chloroplast transgenes.
发表于 2025-3-22 00:38:43 | 显示全部楼层
Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Seeous and unidirectional process. Cytoplasmic sorting allows the segregation of cells with marker-free transgenic plastids. The marker-free shoots resulting from direct repeat mediated excision of marker genes have been isolated by vegetative propagation of shoots in the T. generation. Alternatively,
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发表于 2025-3-22 16:18:46 | 显示全部楼层
Transformation of the Plastid Genome in Tobacco Suspension Cell Culturescells grown in the dark are similar in size and cellular morphology to those in embryogenic culture systems of monocot species, thus providing a useful model for understanding the steps leading to plastid transformation in those recalcitrant species.
发表于 2025-3-22 17:15:10 | 显示全部楼层
1064-3745 Second Edition. is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights .of applications in agriculture, industrial biotechnology and healthcare.  .978-1-0716-1474-7978-1-0716-1472-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-23 00:31:52 | 显示全部楼层
Springer Proceedings in Physics elements and protein factors. This complexity of the plastid transcription apparatus thus provides ample room for regulatory effects at many levels within and beyond transcription. Research in this field offers insight into the various ways in which plastid genes, both singly and groupwise, can be
发表于 2025-3-23 02:22:20 | 显示全部楼层
Suresh Sagadevan,Jiban Podder,Isha Das about the factors influencing the efficiency of plastid transgene expression, and highlight possible optimization strategies to alleviate problems with poor expression levels. I also discuss available techniques for inducible expression of chloroplast transgenes.
发表于 2025-3-23 07:17:43 | 显示全部楼层
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