Titlebook: Chikungunya Virus; Methods and Protocol Justin Jang Hann Chu,Swee Kim Ang Book 2016 Springer Science+Business Media New York 2016 Chikungun

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Book 2016ts, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls..Thorough and practical, .Chikungunya Virus: Methods and Protocols. is auseful and comprehensive resource for CHIKV researchers and anyone interestedin learning more about the Chikungunya Virus..

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Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assayoduct in a reaction (Gibsons et al., Genome Res 6:995–1001, 1996). Here, we describe the use of SYBR Green I-based and TaqMan. real-time reverse transcription polymerase chain reaction (RT-PCR) for the detection and quantification of Chikungunya virus (CHIKV).

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Chikungunya Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluoresceof virus antigen preparation, detection by IFA method, and applications are described. The described IFA method is potentially useful in a wide range of studies including virus growth kinetics and virus infection mechanism studies. Additionally, the described IFA method can be modified for applications in arbovirus diagnosis, including CHIKV.

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1064-3745 ation advice from the experts.Includes supplementary materia.Thisvolume covers multidisciplinary approaches on various aspects of ChikungunyaVirus (CHIKV) research that was brought together from leading laboratoriesacross the globe. Topics covered include techniques in clinical and diagnosticvirolog

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Ausblick: Wohin mit all dem Content?,le humans or among field-caught mosquitoes. This is followed by sequencing a selected region of the virus which will provide evidence on the source of the virus and possible association of the virus to increased cases of Chikungunya infections.