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Titlebook: Chemotaxis; Methods and Protocol Tian Jin,Dale Hereld Book 2016Latest edition Springer Science+Business Media New York 2016 cell movement.s

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发表于 2025-3-21 18:15:40 | 显示全部楼层 |阅读模式
书目名称Chemotaxis
副标题Methods and Protocol
编辑Tian Jin,Dale Hereld
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Chemotaxis; Methods and Protocol Tian Jin,Dale Hereld Book 2016Latest edition Springer Science+Business Media New York 2016 cell movement.s
描述.This second edition expands on the first edition with new chapters describing methods for studyingcell movement, molecular components involved in chemotaxis, spatiotemporaldynamics of signaling components, and quantitative modeling, as well as severalupdated chapters from the first edition. Various methods to investigatedirectional cell growth and movements are presented in Chapters 1-20. Thesechapters contains experimental procedures to visualize and measure migrationbehaviors of different kinds of organisms, including chemotropism in thebudding yeast; cell growth and migration of D. discoideum; border cellmigration in Drosophila; chemotaxis of mouse and human neutrophils; andHIV-induced T cell chemotactic response. .Chemotaxis: Methods and Protocols,Second Edition. also contains microscopy procedures for studying breast cancercell migration, tumor cell invasion in vivo, and axon guidance. The book concludeswith Chapters 21-27 describing methods that measure spatiotemporal dynamics ofsignaling components involved in chemotaxis; introduce imaging techniques, suchas TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models ofexperimentally generated chemoattractant
出版日期Book 2016Latest edition
关键词cell movement; spatiotemporal dynamics of signaling components; quantitative modeling; directional cell
版次2
doihttps://doi.org/10.1007/978-1-4939-3480-5
isbn_softcover978-1-4939-8059-8
isbn_ebook978-1-4939-3480-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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发表于 2025-3-21 20:48:13 | 显示全部楼层
Imaging Polarization in Budding Yeast,fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected aspects of polarization including transient intermedi
发表于 2025-3-22 02:19:11 | 显示全部楼层
Migration of , to the Chemoattractant Folic Acid,es and methods for growing . on a bacterial lawn for several processes studied using this model system. When grown on a bacterial lawn, . show positive chemotaxis towards folic acid (FA). While these vegetative cells are highly unpolarized, it has been shown that the signaling and cytoskeletal molec
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Studying Chemoattractant Signal Transduction Dynamics in , by BRET,uding that of neutrophils to sites of infections and of cancer cells during metastasis. A model frequently used for deciphering chemoattractant signal transduction is the social amoeba .. However, the methods available to quantitatively measure chemotactic signaling are limited. Here, we describe a
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Wave Patterns in Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals,nto a retracting tail. Under the control of chemotactic signal inputs, Ras is activated and PIP. is synthesized at the front, while the PIP.-degrading phosphatase PTEN decorates the tail region. As a result of signal transduction, actin filaments assemble at the front into dendritic structures assoc
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Dissecting Spatial and Temporal Sensing in , Chemotaxis Using a Wave Gradient Generator, healing. Until recently, how cells integrate spatial and temporal information to determine the direction of migration has remained elusive. In ., the chemoattractant cAMP that directs cell aggregation propagates as periodic waves. In light of the fact that any temporally evolving complex signals, i
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Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis,ses many diseases such as cancer, cardiomyopathies, diabetes, microbial infections, and genetic and neurodegenerative disorders. Immunoprecipitation is a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be id
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