Titlebook: Characterization of Plant Viruses; Methods and Protocol Alangar Ishwara Bhat,Govind Pratap Rao Book 2020 Springer Science+Business Media, L

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https://doi.org/10.1007/978-3-642-53162-0iruses. It is also used as a diagnostic tool for detection of viruses, especially of woody plants. Grafting may succeed in transmitting a virus where other methods fail, thus grafting can be used to differentiate plants infected with virus from plants showing virus-like symptoms due to nutritional deficiencies.

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Isolation and Diagnosis of Virus Through Indicator Hosts,e is then made by inoculating single local lesion on to hosts that produce either local or systemic symptoms. Pure culture of the virus is inoculated (either mechanically or through vectors) onto a set of diagnostic hosts that are known to produce characteristic symptoms that would help in the identification of the causal virus.

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Mechanical Sap Transmission,bbing the infectious sap on to the leaf of a healthy plant. The procedure is referred to as mechanical or sap transmission. The different steps and requirements for the process of mechanical transmission are discussed in the chapter.

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