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Titlebook: Characterization of Nanoparticles Intended for Drug Delivery; Jeffrey D. Clogston,Rachael M. Crist,Stephan T. St Book 2024Latest edition L

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书目名称Characterization of Nanoparticles Intended for Drug Delivery
编辑Jeffrey D. Clogston,Rachael M. Crist,Stephan T. St
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Characterization of Nanoparticles Intended for Drug Delivery;  Jeffrey D. Clogston,Rachael M. Crist,Stephan T. St Book 2024Latest edition L
描述This third edition volume expands on the previous editions with new and updated discussions on the latest developments in endotoxin contamination, complex physicochemical properties, in vitro immunotoxicity traits, and in vitro drug release properties. Eight chapters in this book are dedicated to physicochemical characterization techniques and cover newer methods such as asymmetric-flow field-flow fractionation, single particle inductively coupled plasma mass spectrometry, and resistive pulse sensing. The next eighteen chapters explore the immunotoxicity of nanomaterials, including microbial contaminants such as endotoxin and beta-glucans, anti-PEG antibodies, autoimmunity, and immunosuppressive properties. The last two chapters talk about new pharmacology protocols, including a new technique to assess drug release and a tissue distribution assay using PEG immunohistochemistry. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step instructions to reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cut
出版日期Book 2024Latest edition
关键词Nanomedicine; Physicochemical characterization; Immunotoxicity; Nanomaterials; Pharmaceuticals
版次3
doihttps://doi.org/10.1007/978-1-0716-3786-9
isbn_softcover978-1-0716-3788-3
isbn_ebook978-1-0716-3786-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightLeidos Biomedical Research Inc. 2024
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Assessment of Protein Binding Using Asymmetric-Flow Field-Flow Fractionation Combined with Multi-ange nanoparticle and suggests the presence of protein binding. This protocol will discuss how to set up an experiment to assess protein binding in nanoparticles using AF4, multi-angle light scattering (MALS), and dynamic light scattering (DLS).
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Particle Size and Concentration Measurement Using the Spectradyne nCS1 Instrumentnano-constriction in the microfluidic cartridge. This method also has the advantage over optical techniques in that measurements are not dependent on the type of material being measured (e.g., refractive index of the sample itself is not needed for accurate analysis), and only microliters (typically 5 μL) of a sample are needed for analysis.
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Detection of Nanoparticle-Mediated Total Oxidative Stress in T Cells Using CM-H2DCFDA Dye T cells isolated from the peripheral blood of healthy donor volunteers are treated with nanoparticles and controls, and the generation of reactive oxygen species is detected by flow cytometry using CM-H.DCFDA reagent.
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Detection of Induction of Mitochondrial Oxidative Stress by Nanoparticles in T Cells Using MitoSOX Rogy but in other areas of biology as well, including those related to developing novel therapies. Total oxidative stress may result from multiple cellular organelles. The protocol described herein allows for the analysis of oxidative stress in mitochondria.
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