Titlebook: Cereal Genomics; Methods and Protocol Robert J. Henry,Agnelo Furtado Book 2014 Springer Science+Business Media, New York 2014 Genome modifi

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Optimierung für Wirtschaftsingenieure the potential to capture 6–7 kb of sequence in a single round. Ideal for identifying gene promoter regions where only the coding region. Genome walking also has significant utility for capturing homologous genes in new species when there are areas in the target gene with strong sequence conservatio

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Weiss die rechte Hand, was die linke tut?real genomes. The protocol follows a standard approach that has been shown to generate high-quality results: size fractionation of genomic DNA; capillary transfer to a nylon membrane; hybridization with a digoxigenin-labelled probe; and detection using a chemiluminescent-based system. High sensitivi

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https://doi.org/10.1007/978-3-658-27673-7efined which uses helium gas as the propellant to launch alternative heavy metal particles such as gold and silver. The simple principle is that DNA-coated microscopic particles (microcarriers) are accelerated at high speed by helium gas within a vacuum and travel at such a velocity as to penetrate

ANTIC

Considerations for Engineering Practice,ell walls, in order for the introduced genes to be integrated into the host genome. This technique has proven to be an effective and versatile approach towards plant genetic modification in preceding decades. Particle bombardment has been successfully applied to cereals including rice, maize, wheat,

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https://doi.org/10.1007/978-3-658-28199-1 vector with a gene of interest and a selectable marker gene placed between the T-DNA borders; the T-DNA is the region transferred to the plant cells, thus harnessing the bacterium’s natural ability to deliver specific DNA into host cells. Following the initial inoculation with the ., the embryos ar

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