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Titlebook: Cereal Genomics; Methods and Protocol Robert J. Henry,Agnelo Furtado Book 2014 Springer Science+Business Media, New York 2014 Genome modifi

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发表于 2025-3-21 19:46:42 | 显示全部楼层 |阅读模式
书目名称Cereal Genomics
副标题Methods and Protocol
编辑Robert J. Henry,Agnelo Furtado
视频video
概述Is a comprehensive resource for studying cereal genomes.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplemen
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cereal Genomics; Methods and Protocol Robert J. Henry,Agnelo Furtado Book 2014 Springer Science+Business Media, New York 2014 Genome modifi
描述.​In .Cereal Genomics: Methods and Protocols., expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Cereal Genomics: Methods and Protocols. provides a comprehensive resource for those studying cereal genomes..
出版日期Book 2014
关键词Genome modification; analysis of DNA and RNA; cereal genomes; challenging seeds of cereals; disease resi
版次1
doihttps://doi.org/10.1007/978-1-62703-715-0
isbn_softcover978-1-4939-6039-2
isbn_ebook978-1-62703-715-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, New York 2014
The information of publication is updating

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发表于 2025-3-21 22:30:38 | 显示全部楼层
Sandro Bliemetsrieder,Gabriele Fischerkaged grains and to determine the purity of milled grains. Methods outlined here are optimal procedures to isolate DNA from endosperm tissue of modern rice grains and of aged rice remains preserved between 50 and 100 years. The extracted DNA can be used to amplify regions of chloroplast genomic DNA
发表于 2025-3-22 01:39:38 | 显示全部楼层
https://doi.org/10.1007/978-3-658-26256-3riety of forms: total RNA, ribosomal RNA, messenger RNA (mRNA), and small interfering RNA (siRNA) to name a few. In some instances, total RNA is all that is required; however most applications will require the enrichment for some particular form of RNA. In plants, including cereals, total RNA is a m
发表于 2025-3-22 05:07:47 | 显示全部楼层
https://doi.org/10.1007/978-3-658-26256-3be challenging due to this high starch content. Starch coprecipitates with RNA in the presence of isopropanol or ethanol and can interfere with the extraction process and downstream reactions. Thus the removal of starch by using appropriate methods is necessary for obtaining pure RNA to be processed
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https://doi.org/10.1007/978-3-658-26506-9e genomic analysis, complete genome sequencing, and evolutionary studies. Due to their stability and relative simplicity BAC libraries are most preferred over other approaches for cloning large genomic DNA fragments for large-insert libraries. Isolation of intact high molecular weight (HMW) DNA is a
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Dennis Ahrholdt,Goetz Greve,Gregor Hopfgeted induced local lesions in genomes (TILLING) technique has introduced the possibility of reverse genetics—the ability to screen for mutations at the DNA level prior to assessing phenotype. Fundamental to TILLING is the induction of mutant populations (or alternatively, the identification of muta
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