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Titlebook: Cereal Genomics; Methods and Protocol Luis M. Vaschetto Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 High-t

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发表于 2025-3-21 18:06:00 | 显示全部楼层 |阅读模式
书目名称Cereal Genomics
副标题Methods and Protocol
编辑Luis M. Vaschetto
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cereal Genomics; Methods and Protocol Luis M. Vaschetto Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 High-t
描述.The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Authoritative and cutting-edge, .Cereal Genomics: Methods and Protocols .to ensure successful results in the further study of this vital field..
出版日期Book 2020
关键词High-throughput phenotyping; epigenotype-phenotype; Hi-C technique; immunoprecipitation; transcriptomic
版次1
doihttps://doi.org/10.1007/978-1-4939-9865-4
isbn_softcover978-1-4939-9867-8
isbn_ebook978-1-4939-9865-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
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https://doi.org/10.1007/978-3-658-26123-8utine and efficient rice transformation protocols by .. This method uses mature seeds as a material and can be applied to many . and some other varieties of rice. According to this method, it is even possible for beginners to obtain rice transformants.
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Cost-Effective Profiling of , Transposon Insertions in Maize by Next-Generation Sequencing,particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active . elements. Here we describe a rapid and cost-effective Miseq-based . transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.
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Relative Expression Analysis of Target Genes by Using Reverse Transcription-Quantitative PCR,le and efficient protocol to determine gene expression in cereals, based on real-time PCR using SYBR. Green dye. This technique provide an inexpensive alternative, since no probes are required, allowing for the quantification of a high number of genes with reduced cost.
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Transformation in the Rice Genome,utine and efficient rice transformation protocols by .. This method uses mature seeds as a material and can be applied to many . and some other varieties of rice. According to this method, it is even possible for beginners to obtain rice transformants.
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Sequencing and Assembling Genomes and Chromosomes of Cereal Crops,ologies for sequencing and assembly of flow sorted chromosomes and whole genomes in cereal crops, with special emphasis on the case of the International Wheat Genome Sequencing Consortium (IWGSC), are reviewed.
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Genome-Wide Identification of Regulatory DNA Elements in Crop Plants,n both plants and animals. Here we describe a DNase-seq procedure modified and developed for crop plants. These plants usually contain large amounts of repetitive sequences and complex organic constituents. With the main improvement in nuclei isolation, this method has been successfully used in mapping DHSs in cotton and sugarcane.
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