Titlebook: Cereal Genomics; Methods and Protocol Luis M. Vaschetto Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 High-t

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DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics,diated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or

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Genome-Wide Identification of Regulatory DNA Elements in Crop Plants,h sensitive to DNase I digestion, which are termed as DNase I hypersensitive sites (DHSs). These DHSs can be efficiently detected through DNase I digestion followed by high-throughput DNA sequencing (DNase-seq). Thus, DNase-seq has become a powerful technique for DHSs mapping at whole-genome level i

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Genome-Wide Profiling of Histone Modifications with ChIP-Seq, proteins. This protocol has been developed and utilized to perform ChIP on histone covalent modifications in various plant species including cereals. DNA and chromatin-associated proteins are crosslinked with formaldehyde. Chromatin is then isolated from nuclei and sheared via sonication. Antibodie

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