Titlebook: Cellular and Molecular Methods in Neuroscience Research; Adalberto Merighi,Giorgio Carmignoto Book 2002 Springer Science+Business Media Ne

Recess

Transfection Methods for Neurons in Primary Culture,phate (CP) transfection. We have developed this method to analyze localization of proteins in fully mature hippocampal neurons, however it can also be used for others purposes, such as the analysis of gene regulation or function of proteins in neurons. This method offers the advantage of transfectin

巨头

Polyethylenimine: A Versatile Cationic Polymer for Plasmid-Based Gene Delivery in the CNS,able, small (<100 nm ø), and diffusible complexes using a cationic polymer, polyethylenimine (PEI). The approach is extremely versatile as it relies on use of plasmid, not viral-based constructions. This means that construction preparation and amplification is easy to carry out, risk free, and rapid

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sacrum

CANE

绝食

DUCE

cardiovascular

Immunocytochemical Labeling Methods and Related Techniques for Ultrastructural Analysis of Neuronalls within the central nervous system (CNS). According to our current view, neurons communicate to each other mainly at the level of synapses. Although it is now widely accepted that nonsynaptic neuron-to-neuron communication and neuron-to-glia crosstalk also occur, ultrastructural localization of tr

外露

乳汁