Titlebook: Cellular and Molecular Methods in Neuroscience Research; Adalberto Merighi,Giorgio Carmignoto Book 2002 Springer Science+Business Media Ne

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Cellular and Molecular Methods in Neuroscience Research978-0-387-22460-2

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https://doi.org/10.1007/b98863Ethylen; Vivo; cell biology; gene transfer; neurobiology; neurons; neuropeptides; neuroscience; tissue

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978-1-4419-2979-2Springer Science+Business Media New York 2002

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Operation of a Cryogenic Rocket Enginephate (CP) transfection. We have developed this method to analyze localization of proteins in fully mature hippocampal neurons, however it can also be used for others purposes, such as the analysis of gene regulation or function of proteins in neurons. This method offers the advantage of transfectin

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https://doi.org/10.1007/978-3-642-10712-2reatments in living neurons. We produced expression vectors containing chimeras of the green fluorescent protein (GFP)linked to the C terminus of GABA. receptors α1, γ2, or the δ subunits. Human embryonic kidney (HEK) 293 cells were successfully transfected with α1-GFP cDNAs together with β3 subunit

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https://doi.org/10.1007/978-3-642-11434-2 to the advances in neurocytochemichal technologies. First, the identification and in situ localization of neurotransmitters, related enzymes, and receptors greatly benefited from the development of more and more specific and sensitive cytochemical techniques. For instance, after histochemistry of a