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Titlebook: Cellular Imaging; Electron Tomography Eric Hanssen Book 2018 Springer International Publishing AG 2018 3D Reconstruction of Images.Analysi

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Organisationsentwicklung und Fachlichkeitection alignment, and 3D reconstruction. In this chapter we will focus on the process of alignment moving from the justification of its need to the study of the different classical approaches (cross-correlation, use of added fiducial markers) that have been commonly used in this alignment process. W
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Innovation und Beschäftigungsstabilität that the 3D information can be recovered by “back-projecting” all the images in the correct geometry. We use algorithms that are integrative (back-projection or Fourier inversion) or iterative (algebraic or maximum entropy methods). In practical tomography, we can only record a finite set of images
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Astrid Seltrecht Dipl. Päd., Dr.owever, owing to the complexity and low signal-to-noise ratio of the reconstructions, direct interpretation of their three-dimensional content is not straight forward. This chapter describes computational approaches designed to extract the most accurate information possible from biological electron
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https://doi.org/10.1007/978-3-319-68997-53D Reconstruction of Images; Analysis of Cell Structure; Array Tomography; Block Face Tomography; Electr
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Organisationsentwicklung und Fachlichkeitfficiency of descriptive studies of morphology, and in the case of structural biology, to enable the transition from a reductionist approach to in-depth systems biology. The chapter concludes with a step-by-step guide to acquiring tomograms.
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