Titlebook: Cellular Imaging; Electron Tomography Eric Hanssen Book 2018 Springer International Publishing AG 2018 3D Reconstruction of Images.Analysi

装入胶囊

DUCE

Organisationsentwicklung und Fachlichkeitcted using ordinary montaging to ~10 × 10 µm. The desire to study areas larger than this has spurred the development of methods for stitching together laterally adjacent tomograms, referred to as super-montaging. In this chapter, we describe the steps involved in performing large-scale tomography in

Morsel

情感

https://doi.org/10.1007/978-3-531-91117-5Cryo-electron tomography (CET) provides three-dimensional (3D) views on the structure and organization of a wide range of frozen-hydrated specimens under near-to-native conditions. In combination with advanced image processing methods, the locations, orientations and structures of large macromolecular complexes can be mined from tomographic data.

GLADE

Structural Biology in Situ Using Cryo-Electron Subtomogram Analysis,Cryo-electron tomography (CET) provides three-dimensional (3D) views on the structure and organization of a wide range of frozen-hydrated specimens under near-to-native conditions. In combination with advanced image processing methods, the locations, orientations and structures of large macromolecular complexes can be mined from tomographic data.

发展

羽毛长成

https://doi.org/10.1007/978-3-531-90943-1ned samples, and for vitrified, unstained cells. With the proliferation of modern TEM microscopes that allow for switching between TEM and STEM modes with relative ease, we expect the use of STEM tomography to increase. The concepts for STEM imaging are significantly different than for TEM, and ther

WITH

Organisationsentwicklung und Fachlichkeitn uniquely provide mechanistic insights into in situ biology at molecular and cellular scales. To produce these images, specimens are preserved by flash-freezing without stains or fixatives; these flash-frozen specimens are imaged over a range of angles in an electron microscope and this data used t

流行

Feedback

https://doi.org/10.1007/978-3-531-90943-1 main contributors to this are the Serial Block-Face SEM (SBEM) and Focused Ion Beam SEM (FIB-SEM). The resolution offered by the FIB-SEM makes it the go-to tool for studies at higher resolution over smaller areas, particularly for subcellular studies. Compared to FIB-SEM, SBEM gives a much larger f