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Titlebook: Cells and Culture; Proceedings of the 2 Thomas Noll Conference proceedings 2010 Springer Science+Business Media B.V. 2010 Proteomics.bioinf

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Madhura P. Nijsure,Vipuil Kishoremple fluorescence measurement. Subsequently, we cloned genes encoding two main ECM proteins (collagen I and tropoelastin) into mammalian expression vectors. We showed that recombinant elastin and collagen are both expressed and secreted by CHO DG44 cells. In a next step, CHO cells genetically modifi
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https://doi.org/10.1007/978-3-319-73664-8f hMSC at higher population doubling numbers may be of interest due to the requirement of less frequently isolations of cells. Therefore hMSC were cultured in monolayer until the 37th population doubling. Cells of different passages were cultured as pellets for two weeks in transforming growth facto
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al cell technology for industrialists..Practical informationRegeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- niz
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Towards Grounded Enterprise Modellingractivity mainly due to substantial time requirements. However, multiple copies are not limited to gene amplification. Transfection techniques such as calcium phosphate co-precipitation, PEI transfection and even electroporation result in multicopy integrates without the stepwise increase of selection pressure that induce amplification.
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