Titlebook: Cell-free DNA as Diagnostic Markers; Methods and Protocol Valentina Casadio,Samanta Salvi Book 2019 Springer Science+Business Media, LLC, p

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Organised Crime Groups involved in Fraudrting from the hypothesis that the gain of androgen receptor (.) gene is a frequent aberration in advanced prostate cancer patients, we analyzed it in cfDNA as a potential predictive biomarker of specific treatments. Here we report a general protocol that may be considered to analyze gene copy numbe

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Foteini Cheirdari,George Karabatisnalysis of mutations in tumor tissue DNA and plasma cell-free DNA (cfDNA) using a dPCR method. Tumor cells are captured by laser microdissection to obtain only cancerous cells from a small quantity of metastatic tissue samples and to exclude inflammatory and stromal cells. We extracted cfDNA from 50

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Sankalita Mandal,Marcin Hewelt,Mathias Wesken DNA sequence. The mainly studied epigenetic alteration is DNA methylation occurring at CpG islands in the promoter regions for which several literature data showed clinical relevance. This chapter is an overview of the epigenetic alterations detected in cell-free DNA.

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Sankalita Mandal,Marcin Hewelt,Mathias Weskend prediction of therapeutic response. Quantification of cfDNA methylation levels requires methods with high sensitivity and specificity due to low amounts of cfDNA available in plasma, high degradation of cfDNA, and/or contamination with genomic DNA. To date, several approaches for measuring cfDNA

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https://doi.org/10.1007/978-3-319-69505-1tor. Chances of an effective cure in childhood cancer rely on the capacity to make an early and accurate diagnosis, detect metastatic disease or relapse, and predict the response to treatment..Liquid biopsy is a very promising blood test for cancer detection and noninvasive disease monitoring. This

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Lisa E. Flaum,William J. Gradishar way into global clinical applications. The fractional concentration of cell-free fetal DNA in plasma DNA of a pregnant woman is an important parameter for understanding and interpreting analytical results of NIPT. Thus, the accurate quantification of fetal DNA fraction is indispensable when NIPT is

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