Titlebook: Cell-Wide Identification of Metabolite-Protein Interactions; Aleksandra Skirycz,Marcin Luzarowski,Jennifer C. E Book 2023 The Editor(s) (i

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Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution withe. PISA dramatically reduces analysis costs, allowing many research questions in need of target deconvolution to be addressed, and unlocks the potential of miniaturizing biological models, including primary cells.

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Biophysical Approaches for the Characterization of Protein-Metabolite Interactions,tate-of-the-art biophysical toolbox for the study of protein-metabolite interactions. It briefly introduces basic principles, highlights recent examples from the literature, and pinpoints promising future directions.

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,Identification of Plant Protein–Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectroparation is performed including a complete digestion with the sequence-specific protease trypsin. Finally, label-free proteomics analysis is applied to identify structure-dependent proteolytic patterns corresponding to protein targets of the specific metabolite and their binding sites. Given its ame

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Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Diffenteractions of natural products and metabolites, in particular. The reader is also referred to excellent reviews of the field and the literature cited therein. This chapter also provides a detailed experimental protocol for performing the STD NMR measurement based on the example of the subunit A of

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https://doi.org/10.1007/978-3-319-67308-0nteractions of natural products and metabolites, in particular. The reader is also referred to excellent reviews of the field and the literature cited therein. This chapter also provides a detailed experimental protocol for performing the STD NMR measurement based on the example of the subunit A of