Titlebook: Cell-Based Microarrays; Methods and Protocol Ella Palmer Book 2011 Springer Science+Business Media, LLC 2011 Antibody arrays.Functional ana

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Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies,ed at each location. The result is an array whose features are micrometer-sized clusters of cells expressing defined genes. This approach provides for the high-throughput functional analysis of many different proteins in parallel and can be considerably more informative and cost-effective relative to more traditional protein expression techniques.

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Organizations in the Face of Crisiszyme and a fluorescent substrate (ELF-97) to screen for gain-of-function mutations in the type-I cGMP-dependent protein kinase (PRKG1). We have identified a constitutively active mutant of this enzyme in which a conserved Glu at position 81 was mutated to Lys.

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Opium and Empire in Southeast Asiaing on single cell phenotypes, such as systematic localization or co-localization studies or genetic assays for genes affecting probed targets. We demonstrate the use of yeast spheroplast microarrays to probe microtubule and spindle defects across a collection of yeast strains harboring tetracycline-down-regulatable alleles of essential genes.

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G. Donald Chandler III,John W. Chandlerethod based on the production of antibody microarrays specific to two different cell types: B and T lymphocytes. We have also described the real-time monitoring of the cell capture on the microarray using surface plasmon resonance imaging (SPRi).