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Titlebook: Cell-Based Microarrays; Methods and Protocol Ella Palmer Book 2011 Springer Science+Business Media, LLC 2011 Antibody arrays.Functional ana

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发表于 2025-3-21 19:30:50 | 显示全部楼层 |阅读模式
书目名称Cell-Based Microarrays
副标题Methods and Protocol
编辑Ella Palmer
视频videohttp://file.papertrans.cn/223/222931/222931.mp4
概述Provides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand u
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell-Based Microarrays; Methods and Protocol Ella Palmer Book 2011 Springer Science+Business Media, LLC 2011 Antibody arrays.Functional ana
描述As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins.In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for m
出版日期Book 2011
关键词Antibody arrays; Functional analysis; High throughput; Live imaging; Microfluidic image cytometry; Revers
版次1
doihttps://doi.org/10.1007/978-1-61737-970-3
isbn_softcover978-1-4939-5784-2
isbn_ebook978-1-61737-970-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
The information of publication is updating

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Ella PalmerProvides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand u
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Organizations in the Face of Crisis. They reported a technique whereby expression vectors containing the open reading frame (ORF) of human genes were printed onto glass microscope slides to form a microarray. Transfection reagents were added pre- or post-spotting and cells grown over the surface of the array. They demonstrated that c
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Organizations in the Face of Crisisification of gene functions is needed. Reverse transfection microarray technology, which turns a DNA microarray into a cell-based microarray, has emerged for simultaneously studying the function of many genes. Since the initial demonstration in 2001, many variations have surfaced, making the technol
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Organizations in the Face of Crisisassays have employed fluorescent proteins as reporters. These fluorescent reporter proteins can be monitored in living cells but have low sensitivity of detection compared to enzymatic reporters. Here we have described a novel transcriptional reporter assay using the alkaline phosphatase reporter en
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