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Titlebook: Cell Viability Assays; Methods and Protocol Daniel F. Gilbert,Oliver Friedrich Book 2017 Springer Science+Business Media LLC 2017 Misestima

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H. Idrissi,O. Lefebvre,C. Michelott also in the range of tens of milliseconds for excitable cells, such as nerve and muscle. Muscle fibers respond to electrical stimulation with Ca. transients that exceed their resting basal level about 100 times. Fluorescent Ca. dyes have become an indispensable means to monitor Ca. fluctuations in
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H. Idrissi,O. Lefebvre,C. Michelotoccur independently from changes in morphology. Here we describe a method to assess the size of synaptic vesicle pools using live cell fluorescence imaging and a genetically encoded probe (pHluorin). Assessing functional parameters such as the size of synaptic vesicle pools can be a valuable additio
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https://doi.org/10.1007/BFb0085564xt of in vitro neurotoxicity and developmental neurotoxicity testing. Assaying the functional properties of GlyR can serve as an indicator of cellular viability and the integrity of the developing and mature central nervous system. Human pluripotent NTERA-2 (NT2) stem cells undergo neuronal differen
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Elements of nonlinear analysis,arated images in high resolution can be provided while cells are conserved in their native state. Here we describe the seeding of mesenchymal stem cells to bacterial nanocellulose hydropolymer scaffolds followed by 2-channel imaging of cellular autofluorescence (AF) and collagen-I formation using se
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https://doi.org/10.1007/BFb0079929Cell viability assays using multi-well cell culture plates are frequently used for in vitro drug screening. We herein describe an ATP-based luciferase viability assay for animal African trypanosomes using a 96-well plate. This assay could be further applied to the screening of novel compounds for the treatment of animal African trypanosomiasis.
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Assaying Cellular Viability Using the Neutral Red Uptake Assay, ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red after exposure to the xenobiotic under investigation. The neutral red uptake assay is mainly used fo
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