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Titlebook: Cell Viability Assays; Methods and Protocol Daniel F. Gilbert,Oliver Friedrich Book 2017 Springer Science+Business Media LLC 2017 Misestima

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发表于 2025-3-21 19:21:08 | 显示全部楼层 |阅读模式
书目名称Cell Viability Assays
副标题Methods and Protocol
编辑Daniel F. Gilbert,Oliver Friedrich
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Viability Assays; Methods and Protocol Daniel F. Gilbert,Oliver Friedrich Book 2017 Springer Science+Business Media LLC 2017 Misestima
描述.This volume provides an overview of commonly used methods and protocols for cell fitness indicators. Chapters detail biochemical, fluorescence and luminescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and practical, .Cell Viability Assays: Methods and Protocols .aims to ensure successful results in the further study of this vital field..
出版日期Book 2017
关键词Misestimation; enzyme; cell proliferation; Phenotyping; cell imaging
版次1
doihttps://doi.org/10.1007/978-1-4939-6960-9
isbn_softcover978-1-4939-8352-0
isbn_ebook978-1-4939-6960-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media LLC 2017
The information of publication is updating

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K. -H. Hoffmann,E. Jörn,E. Schäfer,H. Weberculate dose-response. The methods would be useful to researchers in preclinical and translational research planning to move away from simplistic monolayer studies and explore 3D spheroid screens for drug safety and efficacy without substantial investment in money or time.
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https://doi.org/10.1007/BFb0077985 and resazurin. They are applicable for adherent or suspended cell lines, easy to perform, and comparably economical. Detailed protocols and notes for easier handling and avoiding pitfalls are enclosed to each assay.
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Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness,to make the inner mitochondrial membrane permeable for protons and allow maximum electron flux through the electron transport chain, and rotenone and antimycin A—to inhibit complexes I and III, respectively. This chapter describes the protocol of OCR assessment in the culture of primary myotubes obtained upon satellite cell fusion.
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Book 2017minescence-based strategies, computational, and label-free methodologies for assaying cellular viability by means of e.g. viscoelastic properties, impedance and multiphoton microscopy. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to th
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