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Titlebook: Cell Surface Receptors; A Short Course on Th Lee E. Limbird Book 1986 Springer Science+Business Media Dordrecht 1986 Hormone.assessment.cel

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书目名称Cell Surface Receptors
副标题A Short Course on Th
编辑Lee E. Limbird
视频videohttp://file.papertrans.cn/223/222881/222881.mp4
图书封面Titlebook: Cell Surface Receptors; A Short Course on Th Lee E. Limbird Book 1986 Springer Science+Business Media Dordrecht 1986 Hormone.assessment.cel
描述The purpose of this book is to serve as a primer for the study of cell surface receptors. The simplified discussion of methods and their underlying prin­ ciples is intended to remove the usual intimidation caused by the specialized vocabulary or sophisticated mathematics that characterize many of the primary papers in this field. In this way, it is hoped that the basic concepts can be emphasized. This book is meant to be a starting point: a textbook as well as a manual to which the investigator can return for a refresher course, when needed. I feit compelled to write this book for several reasons. The primary philosophical reason was to provide, in one volume, an overall perspective on the study ofcell surface receptors that describes their characterization from pharmacological studies in a whole organ or tissue bath (chapter 2) to studies of radioligand binding in isolated membrane and detergent-solubilized pre­ parations (chapters 3-5) and, back again, to studies in intact cells or tissues using morphological and biochemical techniques (chapter 6). It is my opinion that the ultimate understanding of the molecular basis for hormone or drug­ receptor interactions, and the physiolog
出版日期Book 1986
关键词Hormone; assessment; cell; cell surface receptors; cells; membrane; organ; pharmacology; receptor; tissue
版次1
doihttps://doi.org/10.1007/978-1-4757-1882-9
isbn_softcover978-1-4757-1884-3
isbn_ebook978-1-4757-1882-9
copyrightSpringer Science+Business Media Dordrecht 1986
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Identification of Receptors Using Direct Radioligand Binding Techniques,tes for these radiolabeled agents in target tissues where they elicit a physiological effect. This chapter will describe methods for the identification of radioligand binding sites and for obtaining data to establish whether or not the observed binding sites represent the physiological “receptor” fo
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Complex Binding Phenomena, linear transformations of binding data were derived assuming that a reversible bimolecular reaction occurred between ligand and receptor and that this interaction obeyed mass action law, namely *.. Consequently, when data transformations such as the Scatchard plot are nonlinear, when Hill coefficie
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Biochemical Correlates of the Topographical Fate of Ligand-Receptor Complexes,gand and receptor has been conceptualized in a rather static framework, as if the receptor molecule remains unchanged in isolated membrane preparations or in detergent-solubilized solution, even after receptor occupancy. This may or may not be true. However, when ligand binding to intact cells is me
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