Titlebook: Cell Imaging Techniques; Methods and Protocol Douglas J. Taatjes,Jürgen Roth Book 2013Latest edition Springer Science+Business Media, LLC 2

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Spongy-Bone

Image-Based High-Throughput Screening for Inhibitors of Angiogenesis, co-cultured vascular cells form capillary-like networks that model facets of angiogenesis, making it an attractive alternative for anti-angiogenic drug discovery. We have adapted this angiogenesis assay system to a high-throughput format to enable automated image-based high-throughput screening of

露天历史剧

Intravital Microscopy to Image Membrane Trafficking in Live Rats,esigned to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.

Fibrillation

Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent Differential Interfon-fluorescent nanoparticle probes can be identified and tracked dynamically inside crowded living cells with either differential interference contrast (DIC) microscopy or planar illumination microscopy (PIM). The translational and rotational dynamics of anisotropic nanoparticles can be readily extr

florid

,Laser Scanning Cytometry: Principles and Applications—An Update,cytometry (FCM). This review describes attributes of LSC and covers its numerous applications derived from plentitude of the parameters that can be measured. Among many LSC applications the following are emphasized: (a) assessment of chromatin condensation to identify mitotic, apoptotic cells, or se

Prologue

Capture

Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy,ssfully applied to more than one type of lipid study and atomic force microscope (AFM) instrument setup. The basic procedural steps have been used with an Asylum Research MFP-3D BIO and the Bruker (.) BioScope. The AFM imaging protocol has been supplemented by procedures (.) of ellipsometry, standar

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Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy,cle describes the approaches for chemical functionalization of the mica surface. One approach utilizes 3-aminopropyl-trietoxy silane (APTES), enabling one to obtain a smooth surface termed AP-mica. This surface binds nucleic acids and nucleoprotein complexes in a wide range of ionic strengths, in th

拾落穗

Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation, procedures for using AFM indentation to measure the elastic moduli of living cells. We include step-by-step instructions for cantilever calibration and data acquisition using a combined AFM/optical microscope system, as well as a detailed protocol for data analysis. Our protocol is written specific

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Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells,racy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy high-resolution receptor mapping. In this chapter, we provide a flavor of TREC application on vascular endothelial cells by desc