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Titlebook: Cell Fusion; Arthur E. Sowers Book 1987 Springer Science+Business Media New York 1987 Lipid.Mammalia.development.plasma membrane.protein

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Fusion-Permissive Protoplastssed protoplasts/fused + nonfused protoplasts) is greater than 50%. Fusion is calcium stimulated, enhanced by the calcium ionophore, A23187, and inhibited by EGTA and calmodulin antagonists (Boss and Grimes, 1985; Grimes and Boss, 1985).
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The Mechanism of Polyethylene Glycol-Induced Fusion in Model Membranesve PEG from the cell medium by washing. The use of PEG has several additional advantages over other fusogens: reproducibility in fusion capabilities, high fusion efficiency, inexpensiveness, availability, and ease of handling.
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Control of Membrane Fusion in Polyethylene Glycol-Resistant Cell Mutantsing biotechnology industry (Köhler and Milstein, 1975), and it is not surprising that improvements in the efficiency of cell hybridization protocols have been A. major goal in recent years (de St. Groth and Scheidegger, 1980).
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https://doi.org/10.1007/978-1-4757-9598-1Lipid; Mammalia; development; plasma membrane; protein
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978-1-4757-9600-1Springer Science+Business Media New York 1987
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Experimental Cell Fusion with Selected Siphonocladalean Algal Cellswall (Cocking, 1960). With this technique, A. variety of new genetic manipulations have become possible, such as protoplast fusion, uptake of organelles, and uptake of DNA. With the enzymatic digestion procedure, large populations of genetically and physiologically homogeneous protoplasts are now available (Chaleff, 1981).
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