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Titlebook: Cell Cycle - Materials and Methods; Michele Pagano Book 1996 Springer-Verlag Berlin Heidelberg 1996 Apoptosis.DNA.In situ Hybridization.Ki

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https://doi.org/10.1007/978-981-10-8684-7ither intra- or extracellularly, allowing an extremely effective protein purification. Moreover, as it uses an eukaryotic host protein processing, folding and post-translational modifications can occur and, as the host is a yeast cell, maintenance and growth even in high volume (fermentation) are fast, easy and economical.
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Immunofluorescence Techniques in Cell Cycle Studiesay with other factors. Immunofluorescence microscopy allows the study of the abundance and subcellular localization of any protein throughout the cell cycle, and thus provides important information towards an understanding of their role. This technique combines the sensitivity of fluorescent probes
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Synchronization of Mammalian Cellsell synchronization improves conditions by which an actual process under scrutiny can be studied and helps clarify linkage of the process to a particular cell cycle phase transition. In this chapter we will provide methods that have proven effective in synchronizing mammalian cells into defined cell
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