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Titlebook: Cell Cycle - Materials and Methods; Michele Pagano Book 1996 Springer-Verlag Berlin Heidelberg 1996 Apoptosis.DNA.In situ Hybridization.Ki

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,Brauer’s Theorem and Some Applications,Fluorescence in situ hybridization (FISH) is a technique of nucleic acid hybridization that permits the microscopic visualization of a nucleic acid target. FISH is primarily used to map genes to their respective chromosomal locations, but has many other potential applications to the study of human cell cycle genes.
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Fluorescence in Situ Hybridization and Human Cell Cycle GenesFluorescence in situ hybridization (FISH) is a technique of nucleic acid hybridization that permits the microscopic visualization of a nucleic acid target. FISH is primarily used to map genes to their respective chromosomal locations, but has many other potential applications to the study of human cell cycle genes.
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Microinjection and Electroporation of Macromolecules into Live Cellsic inhibitors (e.g., antibodies, synthetic peptides, antisense mRNAs) or activators (e.g., proteins, peptides, cDNAs under the control of a constitutive promoter) has been a useful tool to study the in vivo function of proteins in cell proliferation and cell cycle control.
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Preparation of , Egg Extracts and Their Utilization in Cell Cycle Studies occurs, the embryos containing stores of almost all of the proteins required until the mid-blastula transition. These properties have made the early embryo of . a useful system for dissecting the underlying molecular mechanism controlling the progression of the cell cycle.
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Identification of Novel Cell Cycle Targets Using Small Molecule Ligandshapter by Fiore et al., Part Four). For example, the latter approach was used to identify p21., a small molecular weight inhibitor of cdks (Harper et al. 1993), Cdi1, a dual-specificity phosphatase that interacts with both Cdc2 and Cdk2 (Gyuris et al. 1993; Hannon et al. 1994), and p16., a Cdk4 inhibitor (Serrano et al. 1993).
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