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Titlebook: Cell Cycle Checkpoints; Methods and Protocol Willis X. Li Book 2011 Springer Science+Business Media, LLC 2011 DNA damage checkpoints.apopto

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Closed Loop Control of General Anaesthesiane-345 (S345), which are phosphorylated in active Chk1 molecules, and subsequently dephosphorylated to inactivate Chk1 and allow mitotic entry. Phospho-specific antibodies can be used to detect these activating phosphorylations, and this provides a simple and sensitive marker of Chk1 activation.
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Screening for Radiation Sensitizers of , Checkpoint Mutants,to kill checkpoint mutants preferentially over wild-type. The differential use of wild-type and checkpoint mutants has the potential to identify molecules that act in a genotype-specific manner to eradicate checkpoint mutant tissues when combined with radiation, while sparing wild-type tissues.
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Study of Cell Cycle Checkpoints Using , Cell-Free Extracts,e years have enabled the continued use of the . system to answer a variety of questions in DNA replication, repair and checkpoint signaling. It is anticipated that the versatile . system is amenable to future modification as well to continue studies attempting to understand these important physiological processes.
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,Methods for Studying Checkpoint Kinases – Chk1,ne-345 (S345), which are phosphorylated in active Chk1 molecules, and subsequently dephosphorylated to inactivate Chk1 and allow mitotic entry. Phospho-specific antibodies can be used to detect these activating phosphorylations, and this provides a simple and sensitive marker of Chk1 activation.
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