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Titlebook: Cell Cycle Checkpoints; Methods and Protocol Willis X. Li Book 2011 Springer Science+Business Media, LLC 2011 DNA damage checkpoints.apopto

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发表于 2025-3-21 18:26:35 | 显示全部楼层 |阅读模式
书目名称Cell Cycle Checkpoints
副标题Methods and Protocol
编辑Willis X. Li
视频videohttp://file.papertrans.cn/223/222780/222780.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cell Cycle Checkpoints; Methods and Protocol Willis X. Li Book 2011 Springer Science+Business Media, LLC 2011 DNA damage checkpoints.apopto
描述.Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division. By controlling the orderly progression of critical cell cycle events such as DNA replication and chromosome segregation and ensuring proper repair of damaged DNA, cell cycle checkpoints function to ensure genome integrity. Mechanisms of checkpoint controls are not only the research focus of investigators interested in mechanisms that regulate the cell cycle, but are also the interests of researchers studying cancer development as it is increasingly clear that loss of cell cycle checkpoints, which leads to genomic instability as a result, is a hallmark of tumorigenesis. .Cell Cycle Checkpoints: Methods and Protocols. provides detailed descriptions of methodologies currently employed by researchers in the field, including those commonly used in the mammalian, yeast, .C. elegans, Drosophila., and .Xenopus. model systems. Each chapter describes a specific technique or protocol, such as a method to induce cell cycle checkpoints in a particular model system, to synchronize a population of cells to allow observations of cell cycle progression, to identify genes involved in checkpoint regulati
出版日期Book 2011
关键词DNA damage checkpoints; apoptosis; cell cycle progression; checkpoint kinases; eukaryotic cell division;
版次1
doihttps://doi.org/10.1007/978-1-61779-273-1
isbn_softcover978-1-4939-6181-8
isbn_ebook978-1-61779-273-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC 2011
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Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells,epaired. This is a common response to a variety of DNA damaging agents, including ionizing radiation and many chemotherapeutic agents used in the treatment of cancer. The G2 DNA damage checkpoint acts to inhibit the mitotic cyclin-dependent kinase, and thus cells are arrested in the G2 phase of the
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Evaluating Spindle Assembly Checkpoint Competence in Mouse Oocytes Using Immunoblotting, of the anaphase-promoting complex or cyclosome (APC/C), the SAC sets the timing of anaphase-onset by co-ordinating the timely destruction of key proteins with the completion of chromosome alignment. How mammalian oocytes regulate chromosome segregation during the first meiotic division (meiosis I)
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Studying Cell Cycle Checkpoints Using , Cultured Cells, vitro system instead of a whole organism has two main advantages: it saves time and simple and effective molecular techniques are available. It has been shown that . cells, similarly to mammalian cells, display cell cycle checkpoint pathways required to survive DNA damaging events (de Vries et al.
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Using , Larval Imaginal Discs to Study Low-Dose Radiation-Induced Cell Cycle Arrest,nuing to cycle. . larval epithelial sacs, called imaginal discs, are an excellent in vivo model system for studying radiation-induced cell cycle arrest. Larval imaginal discs go into cell cycle arrest after being subjected to low-dose irradiation, are subject to easy genetic manipulation, are not cr
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