Titlebook: Cell Culture Techniques; Michael Aschner,Lucio Costa Book 2019Latest edition Springer Science+Business Media, LLC, part of Springer Nature

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Radio-Optical Orientation of E/S0 Galaxies This chapter presents a simplified method for preparing brain slices from adult animals. It applies voltage- and current-clamp recordings in slices of the brainstem and cerebellum, two brain regions vital to motor function, for which neurotoxic chemicals have been shown to act. However with slight

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Radio-Optical Orientation of E/S0 Galaxies above with this method. There were no deaths related to the CSF sampling procedure. In conclusion, the present method provides a reliable and reproducible approach for collecting 200 μl CSF in rats without blood contamination.

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https://doi.org/10.1007/978-94-011-5262-4erefore, new, reliable, and efficient screening and assessment tools are needed for better identification, prioritization, and evaluation of chemicals with potential to induce neurotoxicity. A new framework is proposed for the development of a mechanistically informed IATA (Integrated Approaches to

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In Vitro Techniques for Assessing Neurotoxicity Using Human iPSC-Derived Neuronal Models,trophysiology techniques demonstrate that the neuronal networks develop spontaneous activity, including synchronized calcium oscillations that coincide with spontaneous changes in membrane potential as well as spontaneous electrical activity with defined (network) bursting. Importantly, as shown in

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Neuron-Glia Interactions Studied with In Vitro Co-Cultures, spine formation. To this end, this chapter describes the procedures to set up a sandwich co-culture system from primary rat glial cells and hippocampal neurons, and highlights advantages and disadvantages of this approach and its possible application in the investigation of individual glial factor

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Assessment of Mitochondrial Stress in Neurons: Proximity Ligation Assays to Detect Recruitment of Sf signaling cascades on the outer mitochondrial membrane (OMM) can greatly influence organelle form and function. Thus, interpreting OMM signaling events in the context of mitochondrial function is critical to understanding the role of stress-responsive protein kinases and phosphatases in health and

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Epigenetic Changes in Cultures: Neurons and Astrocytes, describe methods for performing primary neuron and primary astrocyte cultures from rodents. We also provide methods for measuring DNA methylation (methylated DNA immunoprecipitation) and histone modifications (chromatin immunoprecipitation). Studies utilizing these methods have resulted in importan