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Titlebook: Celiac Disease; Methods and Protocol Anthony W. Ryan Book 2015 Springer Science+Business Media New York 2015 Coeliac Disease.HLA genotyping

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Study Designs for Exploring the Non-HLA Genetics in Celiac Diseaseonogenic diseases, but common complex polygenic diseases did not prove so tractable..This chapter will describe the genome-wide methods available for genetic analyses of families today and compare these with the previous analyses performed in the 1990s and early twenty-first century.
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Isolation and Cloning of Gluten-Specific T Cells in Celiac Diseaseincluding malnutrition, diarrhea, stomach ache, and failure to thrive. Removal of gluten from the diet eliminates the trigger for these CD4 T cells and leads to recovery. These CD4 T cells thus play a crucial role in the disease pathogenesis. Here we describe how such T cells can be isolated and characterized.
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Book 2015such as next generation sequencing. The book is divided into three parts: Part I gives a background overview with a number of reviews to describe the history and nature of the disease, its diagnosis, the role of animal models, and study designs for investigating genetic susceptibility. Part II descr
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https://doi.org/10.1007/978-1-4939-2839-2Coeliac Disease; HLA genotyping; auto-antigen; gene expression; generation sequencing; non-HLA loci; repor
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978-1-4939-4775-1Springer Science+Business Media New York 2015
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Twenty-Four Hour Ex Vivo Culture of Celiac Duodenal Biopsiesing the disease process without the need for invasive in vivo studies. Biopsies are maintained in an oxygen-rich environment, in contact with, but not submerged in, culture medium. A very straightforward and successful method of organ culture is described here.
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Flow Cytometric Analysis of Human Small Intestinal Lymphoid Cellsonstituents. Improvements in cell isolation techniques in recent years have enabled flow cytometric analyses of cells derived from tissue biopsies. Here we describe a method for isolating and analyzing small intestinal lymphoid cells using flow cytometry.
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Celiac Disease978-1-4939-2839-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
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