Titlebook: Cartilage Tissue Engineering; Martin J. Stoddart,Elena Della Bella,Angela R. Arm Book 2023 The Editor(s) (if applicable) and The Author(s)

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Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes,disease. Despite this, the chondrocyte primary cilium and its associated role in cartilage biology remains poorly understood. Key to elucidating primary cilia structure and function in chondrocytes is the ability to visualize this unique structure. Here we describe materials and methods for immunofl

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Co-culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Sn indirect co-culture technique based on encapsulation of chondrocytes and mesenchymal stromal cells in polyurethane scaffolds and alginate beads, respectively. This way, both cell populations can communicate through paracrine effects in the absence of cell-cell contact. Due to the mechanical proper

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Modulation of MicroRNA Expression During In Vitro Chondrogenesis, proliferation, apoptosis, metabolism, and differentiation. These small non-coding RNAs function by interacting with specific regions in the 3′-untranslated region of mRNAs, thereby resulting in mRNA degradation or suppression of translation. Since miRNAs have the ability to target many mRNAs within

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Histological Preparation and Evaluation of Cartilage Specimens,ed on the importance to investigate cartilage and bone as a unit, which includes the transition zone of the calcified cartilage and tidemark. Reasons for the appropriate selection of histological methods are presented such as when to use (decalcified) specimens for routine paraffin embedding includi

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