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Titlebook: Cartilage Tissue Engineering; Martin J. Stoddart,Elena Della Bella,Angela R. Arm Book 2023 The Editor(s) (if applicable) and The Author(s)

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发表于 2025-3-21 18:15:52 | 显示全部楼层 |阅读模式
书目名称Cartilage Tissue Engineering
编辑Martin J. Stoddart,Elena Della Bella,Angela R. Arm
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cartilage Tissue Engineering;  Martin J. Stoddart,Elena Della Bella,Angela R. Arm Book 2023 The Editor(s) (if applicable) and The Author(s)
描述This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and up-to-date, .Cartilage Tissue Engineering. serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair..
出版日期Book 2023
关键词Cartilage regeneration; Chondrocyte isolation; 3D cartilage models; RNA and protein extraction; Gene tra
版次1
doihttps://doi.org/10.1007/978-1-0716-2839-3
isbn_softcover978-1-0716-2841-6
isbn_ebook978-1-0716-2839-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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发表于 2025-3-21 20:38:19 | 显示全部楼层
Chondrocyte Isolation and Expansion,acted primary chondrocytes will then adhere to standard tissue culture plastics, typically in small clusters, over a period of a few days in monolayer culture. Chondrocyte populations are expanded in a basal medium containing serum, supplemented with ascorbic acid, antibiotics, and sometimes antifun
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Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation, maintenance, and repair. In this chapter, we show how colony-forming progenitor-like cells can be isolated from bovine articular cartilage using differential adhesion to fibronectin. Furthermore, we describe the optimal conditions and factors required to differentiate these progenitor cells to prod
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Physioxic Culture of Chondrogenic Cells, between 2% and 5% oxygen. Although the literature has historically termed this level of oxygen as hypoxia, particularly when doing experiments in vitro in this range, this is actually the physiological oxygen tension experienced in vivo and is more accurately termed physioxia. In general, culture o
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Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering. This makes them ideal candidates for treating focal cartilage defects and cartilage degeneration in osteoarthritis (OA). Observational studies have reported beneficial biological effects of EVs, such as inhibition of inflammation, enhanced extracellular matrix deposition, and reduced cartilage d
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