Titlebook: Cardiac Tissue Engineering; Methods and Protocol Kareen L.K. Coulombe,Lauren D. Black III Book 2022Latest edition Springer Science+Business

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Self-Assembled Heterotypic Cardiac Spheroids from Human Pluripotent Stem Cells,r cell–ECM interactions, analogous to early development. Therefore, this approach creates a 3D platform to study how multicellular heterotypic interactions impact tissue structure, function, and phenotype.

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Defined Engineered Human Myocardium for Disease Modeling , Drug Screening, and Heart Repair,or drug screening) and in vivo (heart repair) application, scale and geometry of tissue engineered heart muscle must be adapted. In this updated methods paper, we report a simple and scalable (up and down) collagen-based protocol for the construction of Engineered Human Myocardium (EHM) under defined, serum-free conditions.

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Book 2022Latest editionnts in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New animal models, biomaterials, and quantitative analyses are described for broad adoption. Written for the highly successful .Methods in Molecular Biology. series, c

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1064-3745 expertsThis detailed volume presents an updated collection of state-of-the-art protocols in cardiac tissue engineering. These protocols demonstrate advancements in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New anima

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https://doi.org/10.1007/978-3-319-46150-2he genes to be modified can be curated or whole genomes can be investigated. The use of lentiviral sgRNA delivery followed by high-throughput sequencing allows for rapid comparison and identification of candidate genes and epigenetic modifiers, which can be further validated individually or in sub-pooled libraries following screening.