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Titlebook: Cardiac Tissue Engineering; Methods and Protocol Kareen L.K. Coulombe,Lauren D. Black III Book 2022Latest edition Springer Science+Business

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发表于 2025-3-21 19:32:26 | 显示全部楼层 |阅读模式
书目名称Cardiac Tissue Engineering
副标题Methods and Protocol
编辑Kareen L.K. Coulombe,Lauren D. Black III
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cardiac Tissue Engineering; Methods and Protocol Kareen L.K. Coulombe,Lauren D. Black III Book 2022Latest edition Springer Science+Business
描述This detailed volume presents an updated collection of state-of-the-art protocols in cardiac tissue engineering. These protocols demonstrate advancements in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New animal models, biomaterials, and quantitative analyses are described for broad adoption. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Cardiac Tissue Engineering: Methods and Protocols, Second Edition. serves as an ideal resource for inspiring the advancement of cardiotoxicity assessment, drug discovery, and heart repair and regeneration in order to accelerate heart health improvement around the globe..
出版日期Book 2022Latest edition
关键词Heart tissue; Personalized medicine; 3-Dimensional engineered microenvironments; Cardiomyocytes; Cardiac
版次2
doihttps://doi.org/10.1007/978-1-0716-2261-2
isbn_softcover978-1-0716-2263-6
isbn_ebook978-1-0716-2261-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2022
The information of publication is updating

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Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electial infarction (MI) treatment. Here, we described an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae, myocardial slices as a biomimetic cell culture and delivery platform, and a multi-stimulation bioreactor that is able to prov
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,FRESH 3D Bioprinting a Ventricle-like Cardiac Construct Using Human Stem Cell-Derived Cardiomyocytee embedding of suspended hydrogels (FRESH) 3D bioprinting. To do this, we print a support structure using a collagen type I ink and a cellular component using a high-density cell ink supplemented with fibrinogen. The gelation of the collagen and the fibrinogen into fibrin is initiated by pH change a
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Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Plurivides a useful platform for in vitro cardiac development and disease modeling. This protocol describes the generation of fibrin-based engineered heart tissues (EHTs) containing hiPSC-CMs and human stromal cells. The platform makes use of racks of silicone posts that fit a standard 24-well dish. Stro
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High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyes are seeded to form a cell monolayer on a field of sensors able to detect electrical signals, called extracellular field potentials (EFPs). This noninvasive tool allows researchers to investigate key parameters such as EFP amplitude, duration, and arrhythmias. MEA is progressively considered the g
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,Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues,g a focus on single select ion channels, the use of non-human species in vitro and in vivo, and limited direct physiological translation. To be predictive of actual adverse clinical arrhythmic risk, arrhythmia assessment models for chemicals and drugs should be fit-for-purpose and suited for evaluat
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Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling,forms of cardiac disease and has a direct deleterious effect on cardiac function. As currently there is no effective therapeutic strategy specifically designed to target fibrosis, in vitro diagnostic platforms for drug testing have generated significant interest. In this context, we have developed a
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