Titlebook: Cardiac Gene Therapy; Methods and Protocol Kiyotake Ishikawa Book 2017 Springer Science+Business Media New York 2017 Vector technologies.Ca

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Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV) low immunogenicity relative to other vectors and high and long-term transduction efficiency. This field has also been hampered by the presence of preexisting neutralizing antibodies, not only in patients participating in clinical trials but also in preclinical large animal models. These conflicting

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Synthesis of Modified mRNA for Myocardial Deliverym used in vitro or in vivo to achieve transient expression of therapeutic proteins in a heterogeneous population of cells. Incorporation of specific modified nucleosides enables modRNA to be translated efficiently without triggering antiviral and innate immune responses. ModRNA has been shown to be

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Exosomes-Based Gene Therapy for MicroRNA Deliveryveral treatment strategies using drugs and surgeries are being developed for cardiovascular manifestations, gene-based therapies hold significant promise. Recent findings from our laboratory unveiled a novel mechanism that exosomes, secreted nanovesicles from stem cells, mediate cardiac repair via t

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Gene Transfer in Isolated Adult Cardiomyocytes in non-dividing cells, viral transduction has become the method of choice for efficient gene transfer into cardiomyocytes. However, in vitro gene delivery is dependent on a robust cell isolation protocol, as prolonged cultivation is needed to initiate gene expression and target specific cellular pr

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Gene Transfer in Cardiomyocytes Derived from ES and iPS Cellse used as a platform to study cardiac diseases and to explore new therapies..The ability to genetically manipulate hiPSC-CMs not only is essential for identifying the structural and/or functional role of a protein but can also provide valuable information regarding therapeutic applications. In this

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