Titlebook: Cardiac Gene Therapy; Methods and Protocol Kiyotake Ishikawa Book 2017 Springer Science+Business Media New York 2017 Vector technologies.Ca

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Silencing Genes in the Heartre the inducers of RNAi, but cultured primary cardiomyocytes and heart are highly resistant to siRNA transfection. This can be overcome by delivery of small hairpin (sh)RNAs or artificial microRNA (amiRNAs) by cardiotropic adeno-associated virus (AAV) vectors. Here we describe as example of the sile

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Generation of Efficient miRNA Inhibitors Using Tough Decoy ConstructsNAs known as microRNAs (miRNAs), and this mechanism is utilized by organisms ranging from plants to humans. MiRNAs are important downregulators of gene expression and are seen to be dysregulated in disease development. Thus inhibition of aberrantly upregulated miRNAs as a therapeutic approach has be

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Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarctionmmon and morbid disease that is usually caused by irreversible loss of functional cardiomyocytes (CMs). Recently, we and others showed that in a murine model of acute myocardial infarction, delivery of three transcription factors, Gata4, Mef2c, and Tbx5 converted endogenous cardiac fibroblasts into

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Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9temic or regional vector delivery. In this chapter, we describe the most widely used production and purification method of AAV9. This production approach does not depend on the use of a helpervirus but instead on transient transfection of HEK293T cells with a plasmid containing the recombinant AAV g