Titlebook: Capillary Electrophoresis-Mass Spectrometry; Methods and Protocol Christian Neusüß,Kevin Jooß Book 2022 Springer Science+Business Media, LL

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1064-3745 ation advice from the experts.This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, a

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History of DNA Sequencing Technologiesr, we describe in detail the strategy for the targeted screening of modified amino acids (MAAs), using as an example .-proline and its modified derivatives. We illustrate the strategy with two case studies in human plasma.

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Top-Down Proteomics by Capillary Zone Electrophoresis-Tandem Mass Spectrometry for Large-Scale Charpillaries, a dynamic pH junction sample stacking method, a commercialized electro-kinetically pumped sheath flow CE-MS interface and an Orbitrap mass spectrometer. CZE-MS/MS can identify hundreds of proteoforms routinely from the . sample with a 1% proteoform-level false discovery rate (FDR).

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,Assessing the Energy Status of Low Numbers of Mammalian Cells by Capillary Electrophoresis–Mass Speng with cell cultures is the adenylate energy charge (AEC), which represents an index of the energy state of a cell. In this protocol, a CE-MS strategy is proposed for the reliable determination of the adenylate energy charge (AEC) in metabolomics studies dealing with low numbers of mammalian cells.

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,Protocol for Etching Bare-Fused Silica Capillaries for Sheathless Capillary Electrophoresis–Mass Sption of a suitable capillary for the porous tip sprayer is more challenging. The porous tip is created by etching the bare-fused silica capillary with hydrofluoric acid. Here we describe the complete process of etching bare-fused silica capillaries, from length cutting to quality control of the newly etched capillary.

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